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H3R26me2s Antibody
SKU: orb1257936
Featured
Description
Images & Validation
−Item 1 of 9
| Tested Applications | IF, WB |
|---|---|
| Reactivity | Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding Arg26 of human histone H3 |
| Target | H3R26me2s |
| Molecular Weight | Observed: 17kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H3/f, H3/h
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Histone H3R26me2s Antibody [orb191887]
IF, WB
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50 μl, 100 μl
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Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936).
Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
H3R26me2s
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
H3R26me2s Antibody (orb1257936)
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