TbHsp70 Antibody (HRP)

SKU: orb535203

Description

Rabbit polyclonal antibody against TbHsp70 conjugated to HRP

Research Area

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

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Tested Applications	WB
Dilution range	WB (1:1000)
Reactivity	Plant
Application Notes	#REF!

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide from the mid-protein of Trypanosoma brucei brucei TbHsp70 (aa. 200-300)
Target	TbHsp70
Molecular Weight	71 kDa
Purification	Peptide Affinity Purified
Conjugation	HRP

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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TbHsp70, Heat shock 70 kDa protein 4, HSP70-4

Quality Guarantee

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Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Rabbit Anti-TbHsp70 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37°C. Lane 3: Cells incubated for 1 hour at 42°C. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TbHsp70 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71 kDa.

Quick Database Links

Gene Symbol

TbHsp70

UniProt

P11145

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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