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TGFβ RI (phospho Ser165) rabbit pAb
SKU: orb770253
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Description
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−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution range | WB 1:500-2000, IF 1:50-300, IHC 1:50-300 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TGF beta Receptor I around the phosphorylation site of Ser165. AA range:131-180 |
| Molecular Weight | 56kD |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-TGFBR1 antibody, anti-ALK5 antibody, anti-SKR4 antibody, anti-TGF-beta receptor type-1 antibody, anti-TGFR-1 antibody, anti-Activin A receptor type II-like protein kinase of 53kD antibody, anti-Activin receptor-like kinase 5 antibody, anti-ALK-5 antibody, anti-ALK5 antibody, anti-Serine/threonine-protein kinase receptor R4 antibody, anti- antibody
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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, TGFβ RI (phospho Ser165) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, TGFβ RI (phospho Ser165) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, TGFβ RI (phospho Ser165) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western Blot analysis of MOUSE-LIVER cells using Phospho-TGFβ RI (S165) Polyclonal Antibody diluted at 1:1000.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
TGFβ RI (phospho Ser165) rabbit pAb (orb770253)
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