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TIGIT Antibody
SKU: orb1240140
Featured
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 8
| Tested Applications | ELISA, FC, ICC, IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 4A11 |
| Immunogen | TIGIT antibody was raised against the extracellular domain of human TIGIT |
| Target | TIGIT |
| Molecular Weight | Predicted: 26 kDa Observed: 47 kDa |
| Purification | TIGIT Antibody is supplied as protein A purified IgG1. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | TIGIT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
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Western blot analysis of TIGIT in over expressing HEK293 cells using orb1240140 antibody at (A) 0.25 μg/ml, (B) 0.5 μg/ml, and (C) 1 μg/ml.
Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml. Green: TIGIT Antibody [4A11] (orb1240140) Blue: DAPI staining
Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml. Green: TIGIT Antibody [4A11] (orb1240140) Blue: DAPI staining
Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.
Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.
Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA with orb1240147, orb1240136, orb1240137, orb1240140, orb1240146 and orb1240115 antibodies at decreasing concentrations.
A sandwich ELISA was performed using the anti-TIGIT mAb orb1240140 (5 μg/ml) as the capture antibody. Biotin-labeled anti-TIGIT mAb orb1238175 (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 40 pg.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
TIGIT Antibody (orb1240140)
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