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TIM3 antibody

SKU: orb2276373

Description

TIM3 antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsIHC
Dilution Range1:100-1:200
ReactivityHuman
Application Notes
Positive Control: Tonsil: A moderate to strong TIM-3 immunostaining should be seen in a significant fraction of T-cells, while only few TIM-3 positive cells are seen in the germinal centre and the mantle zone. Negative Control: Tonsil: Epithelial cells should be negative. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

HostRabbit
ClonalityMonoclonal
IsotypeIgG
Clone No.MSVA-366R
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD366; HAVR2; Hepatitis A virus cellular receptor 2 (HAVCR2); Kidney injury molecule 3 (KIM3); T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM3; TIMD4

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TIM3 antibody

Alveolar macrophages show strong TIM 3 immunostaining.

TIM3 antibody

Colon descendens, mucosa: In the colon mucosa, variable levels of TIM-3 immunostaining are seen in T-cells and in macrophages. Epithelial staining does not occur.

TIM3 antibody

Uterus cervix TIM3 negative squamous cell carcinoma showing a large number of TIM3 positive macrophages and also lymphocytes.

UniProt Details

No UniProt data available

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Protocol Information

IHC
Immunohistochemistry
View Protocol

TIM3 antibody (orb2276373)

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Available Sizes

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0.5 ml
¥ 31,330.00
Free Secondary Antibody (20 ul)0/0

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