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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mTNF-α (murine TNF-alpha). |
| Target | Tnf |
| Purification | Anti-mTNF-α specific antibody was purified by affinity chromatography and then biotinylated. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tnfsf2, Tumor necrosis factor, Cachectin
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To detect Murine TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Murine TNF-alpha (orb1272285) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine TNF-alpha.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Tnf Antibody (Biotin) (orb1272284)
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