Recombinant TNFa Antibody / Rabbit Monoclonal

SKU: orb606510

Description

This mAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Research Area

Epigenetics, NF-kB, Neuroscience, Signaling Pathways

Images & Validation

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Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 4-8ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant TNFa antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	TNF/1500R
Immunogen	Recombinant human protein was used as the immunogen for the recombinant TNFa antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Recombinant TNFa Antibody / Rabbit Monoclonal

IHC testing of FFPE human pancreas with recombinant TNFa antibody (clone TNF/1500R). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE human histiocytoma with recombinant TNFa antibody (clone TNF/1500R). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant TNFa antibody (clone TNF/1500R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P01375

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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