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TNF alpha Rabbit Polyclonal Antibody
SKU: orb1294347
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500-1:1500, Immunofluorescence 1:200-1:300, Immunohistochemistry (Paraffin) 1:100-1:200 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Recombinant fragment corresponding to Human TNF alpha. |
| Target | TNF alpha |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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anti-TNF alpha antibody at 1/700 dilution, Lysates at 50 µg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294347 overnight at 4°C.
anti-TNF alpha antibody at 1/700 dilution, Lysates at 50 µg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294347 overnight at 4°C.
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294347 anti-TNF alpha antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling TNF alpha with orb1294347 at 1/150.
Quick Database Links
Gene Symbol
TNF alpha
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
TNF alpha Rabbit Polyclonal Antibody (orb1294347)
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