TNF-RII, Human, mAb MR2-1, FITC Antibody

SKU: orb1109059

This product can be custom made

Description

Mouse monoclonal antibody against TNF-RII conjugated to FITC

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications	FC, Functional Studies, IA, IF, IP, WB
Dilution Range	It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Advised positive controls for frozen sections are human lymphnodes and for flow cytometry PHA activated T cells.
Reactivity	Human
Application Notes	IA: It can be used as a capture antibody. IP: The lysate was centrifuged and the supernatant was incubated with 5 mg TNFR2-specific antibody MR2-1 for 2 hours at 4°C. The immuno complexes were precipitated with protein G agarose for two hours at 4°C (Ref.4). W: A reduced protocol was used. The expected band size is ~65 kDa.

Key Properties

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Clonality	Monoclonal
Isotype	Mouse IgG1
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	1 ml (100 µg/ml) 0.2 um filtered protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Buffer/Preservatives	20 um filtered in PBS+1%rAlbumin+0.02%NaN3
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence

Immunoprecipitation