TNF-RII, Mouse, mAb HM102 Antibody

SKU: orb1109195

This product can be custom made

Description

Mouse monoclonal antibody to TNF-RII

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications	FC, Functional Studies, IA, IHC-Fr, IP, WB
Dilution Range	Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
Reactivity	Mouse
Application Notes	FS: In most cases 2µg/ml acts agonistically FC: 100.000 microglia cells were incubated with 10µg/ml PBS/1%serum for 30 min on ice W: A reduced sample treatment and SDS-Page was used. The band size (s) is 75 kDa (Ref.4).

Key Properties

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Clonality	Monoclonal
Isotype	Rat IgG2a
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	1 ml (100 µg/ml) 0.2 um protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin.
Buffer/Preservatives	Quantities >500 µg will be sent without preservatives and are carrier-free.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Tumor necrosis factor receptor superfamily member 1B, CD120b, TNF-R2, p75, p80 TNF-alpha receptor Gene name: Tnfrsf1b

Quality Guarantee

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunoprecipitation