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Toll-like Receptor 7 Rabbit Polyclonal Antibody

SKU: orb1294285

Description

Rabbit polyclonal antibody to Toll-like Receptor 7

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsIF, IHC, WB
Dilution RangeWestern Blot 1:500-1:1500, Immunofluorescence 1:200-1:400, Immunohistochemistry (Paraffin) 1:200-1:300
ReactivityHuman, Mouse, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit-IgG
ImmunogenSynthetic peptide / Intracellular domain corresponding to Human Toll-like Receptor 7 / TLR7
TargetToll-like Receptor 7 / TLR7
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Toll-like Receptor 7 Rabbit Polyclonal Antibody

anti-Toll-like Receptor 7 antibody at 1/800 dilution, Lysates at 40 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294285 overnight at 4°C.

Toll-like Receptor 7 Rabbit Polyclonal Antibody

Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294285 anti-Toll-like Receptor 7 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Toll-like Receptor 7 Rabbit Polyclonal Antibody

Immunohistochemical analysis of paraffin embedded mouse testis labeling Toll-like Receptor 7 with orb1294285 at 1/200.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

Toll-like Receptor 7 Rabbit Polyclonal Antibody (orb1294285)

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Available Sizes

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25 μl
¥ 3,380.00
100 μl
¥ 5,070.00
DispatchUsually dispatched within 5-10 working days
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