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TOP1 Antibody (N-term)
SKU: orb1936312
Description
Research Area
Cancer Biology, Infectious Disease & Virology
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | WB - 1:1000, IF - 1:10-50 |
| Reactivity | Human |
| Predicted Reactivity | Hamster, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 90726 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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TOP1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the TOP1 antibody detected the TOP1 protein (arrow).
Confocal immunofluorescent analysis of TOP1 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
TOP1 Antibody (N-term) (orb1936312)
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