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TP53 Antibody
SKU: orb1336382
Description
Images & Validation
−Item 1 of 8
| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Dilution range | WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100 |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell. |
| Target | TP53 |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Disclaimer | For research use only |
Alternative Names
−BCC7; LFS1; P53; TRP53
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)
Anti-TP53 mouse monoclonal antibody (TA502780) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53)
HEK293T cells transfected with either RC200003 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TP53 antibody (TA502780), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-TP53 antibody (TA502780), (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-TP53 antibody (TA502780), (Red), compared to a nonspecific negative control antibody, (Blue).
Quick Database Links
Gene Symbol
TP53
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
TP53 Antibody (orb1336382)
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