Traumatic Acid is a product of the hydroperoxide lyase pathway in plants. Potential as a wound healing agent that stimulates cell division near a wound site to form a protective callus.
Images & Validation
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Key Properties
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CAS Number
6402-36-4
MW
228.28
Purity
>98% (HPLC)
Formula
C12H20O4
SMILES
C(CCCCC(=O)O)CCC/C=C/C(=O)O
Target
Others
Solubility
In Vitro: DMSO : 250 mg/mL (1095.15 mM)
Bioactivity
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In Vitro
Traumatic Acid (0.1, 1 μM; 5 days) significantly increases cell number in fibroblasts. Traumatic Acid (0.1, 1 μM; 5 days) increases content of GPX activity and reduced glutathione, as well as decreases membrane phospholipid peroxidation in fibroblasts. Traumatic Acid (0.1, 1 μM; 5 days) enhances the production and secretion of medium collagen in medium of fibroblasts. Traumatic Acid (100, 200, 400, 600 μM; 48 h) significantly decreases live cell number, especially after 48h treatment at 100 μM and 200 μM in MCF-7 cells. Traumatic Acid (50-600 μM; 24, 48 h) causes dose-and time-dependent reduction in cell viability and induces apoptosis in MCF-7 cells. Traumatic Acid (50-200 μM; 24, 48 h) results in an oxidative damage of protein in MCF-7 cells. Traumatic Acid (100, 200 μM; 24, 48 h) efficiently enhances oxidative stress level in MCF-7 cells. Cell Proliferation Assay Cell line: Fibroblasts. Concentration: 0.1, 1 μM Incubation time: 5 days. Result: Caused a significant increase in cell number, especially on day 1 at a concentration of 1 μM. Increased cell number of 133 % and 118 % compared to the untreated control cells for concentrations of 1 and 0.1 μM, respectively. Cell Viability Assay Cell line: Fibroblasts. Concentration: 0.1, 1 μM Incubation time: 5 days. Result: Increased total protein content of 183 % and 90% compared to the control at concentrations of 1 and 0.1 μM on day 1.Increased collagen content of 72 % at 0.1 μM (on the day 3) and of 51 % at 1 μM (on the day 1) compared to the control. Increased GPX activity by 111 % and 97 % at concentrations of 1 and 0.1 μM compared to the control. Increased content of reduced glutathione of 86 % and 80% at 0.1 and 1 μM, respectively. Decreased membrane phospholipid peroxidation. Cell Viability Assay Cell line: MCF-7 cells. Concentration: 100, 200, 400, 600 μM. Incubation time: 48 h. Result: Decreasd live cell number of about 76% at 100 μM concentration. Cell Viability Assay Cell line: MCF-7 cells. Concentration: 50-200 μM. Incubation time: 24, 48 h. Result: Increased thiol group content of 167% at 100 μM and 24 h. Cell Viability Assay Cell line: MCF-7 cells. Concentration: 100, 200 μM. Incubation time: 24, 48 h. Result: Increased the amount of ROS. Apoptosis Analysis Cell line: MCF-7 cells. Concentration: 50-600 μM. Incubation time: 24, 48 h. Result: Increased level of apoptosis in a time- and dose-dependent manner.
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