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TRC051384

SKU: orb1304119

Description

TRC051384 is a small molecule HSP70 inducer that activates HSF1, elevating chaperone and anti-inflammatory activity. It has demonstrated neuroprotective effects in a rat transient ischemic stroke model, increasing neuronal Hsp72 expression and survival. This compound is a valuable research tool for studying heat shock response mechanisms in both in vivo and in vitro models of neuroinflammation and ischemia.

Research Area

Metabolism Research, Signal Transduction

Images & Validation

Key Properties

CAS Number867164-40-7
MW465.54
Purity98.79%
FormulaC25H31N5O4
SMILESO=C(NCCN1CCOCC1)Nc1ccc(cc1)C(=O)\C=C\c1cccc(n1)N1CCOCC1
TargetHSP
SolubilityDMSO:16.67 mg/mL (35.81 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.3 mM)

Bioactivity

In Vivo
Administering TRC051384 significantly diminishes stroke-related neuronal damage and disability in a rat model of transient ischemic stroke, achieving an 87% reduction in the area of the penumbra recruited to infarct and a 25% reduction in brain edema, even when treatment commences 8 hours after ischemia onset. Additionally, initiating TRC051384 treatment 4 hours post-ischemia onset notably enhances survival rates, with a 50% improvement by day 2 and a 67.3% increase by day 7. The mechanism underlying TRC051384's efficacy involves the induction of HSP70 through HSF1 activation, which amplifies chaperone and anti-inflammatory activities.
In Vitro
TRC051384 significantly enhances HSP70B mRNA expression hundreds of times in both HeLa cells and rat primary mixed neurons in a dose-dependent fashion. Additionally, it markedly boosts HSF1 transcriptional activity and luciferase recovery upon treatment, following a dose-responsive pattern. Furthermore, TRC051384 demonstrates substantial inhibitory effects on LPS-induced TNF-α expression in the differentiated THP-1 cell line, achieving 60% inhibition at 6.25 μM and 90% inhibition at 12.5 μM .
Cell Research
HeLa cell transiently co-transfected with heat shock elements-luciferase reporter and normalization vector, β-galactosidase are treated with vehicle or TRC051384 (12.5 and 25 μM) for 4 hours. Cell lysates are then prepared and analyzed for luciferase and β-galactosidase activity.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

inhibit, HSP70, Inhibitor, Heat shock proteins, HSP, TRC051384, TRC-051384, TRC 051384

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Quality Guarantee

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Key Properties

No computed properties available.

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Protocol Information

TRC051384 (orb1304119)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

1 mg
¥ 1,040.00
1 ml x 10 mM (in DMSO)
¥ 1,560.00
5 mg
¥ 1,560.00
10 mg
¥ 2,080.00
25 mg
¥ 3,510.00
50 mg
¥ 4,680.00
100 mg
¥ 6,500.00
200 mg
¥ 9,100.00
DispatchUsually dispatched within 3-5 working days
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