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Tri-Methyl-Histone H3 (Lys4) Rabbit Polyclonal Antibody
SKU: orb1172869
Description
Research Area
Epigenetics and Nuclear Signaling
Images & Validation
−Item 1 of 2
| Tested Applications | ChIP, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200, ChIP=1:100-500 |
| Reactivity | Human, Monkey, Mouse, Rat |
| Predicted Reactivity | Monkey, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target | H3C1 |
| Purification | Antigen affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
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Quality Guarantee
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: HeLa, C2C12, COS-7, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.
Tissue: Mouse colon, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:500, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172869.
Quick Database Links
Gene Symbol
H3C1
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
ChIP
Chromatin Immunoprecipitation
Tri-Methyl-Histone H3 (Lys4) Rabbit Polyclonal Antibody (orb1172869)
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