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TriMethyl-Histone H3 (Lys27) monoclonal antibody
SKU: orb1723266
Description
Images & Validation
−
| Tested Applications | ChIP, ICC, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20 |
| Reactivity | Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Immunogen | A synthetic methyl-peptide corresponding to residues surrounding Lys27 of human Histone H3 |
| Molecular Weight | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−H3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
ICC
Immunocytochemistry
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation
TriMethyl-Histone H3 (Lys27) monoclonal antibody (orb1723266)
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