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Trypsin Inhibitor Antibody Biotin Conjugated
SKU: orb344979
Description
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| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500 |
| Reactivity | Plant |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor. |
| Purity | Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soy Bean]. Cross reactivity against Trypsin Inhibitor from other sources is unknown. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-trypsin inhibitor antibody biotin conjugation, biotin conjugated rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody
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Western Blot of Rabbit anti-Soybean Trypsin Inhibitor antibody. Lane 1: ~1 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 2: 0.25 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 3: ~1 ug of purified Soybean Trypsin Inhibitor Non-reduced. Lane 4: 0.25 ug of purified Soybean Trypsin Inhibitor Non-reduced. Primary antibody: Soybean Trypsin Inhibitor antibody at 1:5000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Streptavidin, 1:5K and Atto 425 conjugated goat anti rabbit, 1:10K at 1.5 hr at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 24 kDa, 17 kDa for Trypsin. Other band(s): none.
Western Blot of Rabbit anti-Soybean Trypsin Inhibitor antibody. Lane 1: Soybean Trypsin Inhibitor Reduced. Lane 2: Soybean Trypsin Inhibitor Non-Reduced. Lane 3: Streptavidin Reduced. Lane 4: Streptavidin Non-reduced. Load: ~1 ug per lane. Primary antibody: 1:1000 dilution of primary antibody (ON 4 C). Secondary antibody: Dylight 649 conjugated Donkey anti rabbit (1:10K 1.5 hr RT). Block: orb348637 overnight at 4°C. Predicted/Observed size: 24 kDa/20kDa for Trypsin, 18.8 kDa/15 kDa for Streptavidin. Other band(s): none.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Trypsin Inhibitor Antibody Biotin Conjugated (orb344979)
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