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TUBB3 Antibody (N-term)
SKU: orb1930373
Description
Images & Validation
−Item 1 of 5
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Gallus, Monkey, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 50433 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−TUBB4
Quality Guarantee
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TUBB3 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TUBB3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TUBB3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
TUBB3 Antibody (N-term) western blot analysis in HepG2 cell line lysates (15 ug/lane). This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).
TUBB3 Antibody (N-term) western blot analysis in mouse brain tissue lysates (15 ug/lane). This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).
All lanes: Anti-TUBB3 Antibody (N-term) at 1:1000 dilution. Lane 1: human brain lysate. Lane 2: human cerebellum lysate. Lane 3: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
TUBB3 Antibody (N-term) (orb1930373)
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