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Ubiquitin Antibody (Biotin)
SKU: orb396237
Description
Research Area
Neuroscience, Protein Biochemistry, Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, ICC, IF, IHC, IP, WB |
|---|---|
| Dilution range | WB (1:10000) |
| Reactivity | All |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | FK2 |
| Immunogen | Ubiquitin conjugated lysozyme |
| Target | Ubiquitin |
| Purification | Protein A Purified |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Conjugated antibodies should be stored according to the product label |
|---|---|
| Buffer/Preservatives | 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | BAC56955.1 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Ubiquitin Antibody (Biotin) (orb396237)
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