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Ubiquitin Antibody (PerCP)
SKU: orb699408
Description
Research Area
Neuroscience, Protein Biochemistry, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, ICC, IF, IHC, WB |
|---|---|
| Dilution range | WB (1:10000), ICC/IF (1:100) |
| Reactivity | All |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone No. | FK1 |
| Immunogen | Ubiquitin conjugated lysozyme |
| Target | Ubiquitin |
| Purification | Gel filtration |
| Conjugation | PerCP |
Storage & Handling
−| Storage | Conjugated antibodies should be stored according to the product label |
|---|---|
| Buffer/Preservatives | 95.46mM Phosphate, 2.48mM MES and 2mM EDTA |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.
Western Blot analysis of Poly-Ubiquitin showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1. Lane 1: MW ladder. Lane 2: Poly-Ubiquitin, K48-linked (2 ug). Lane 2: Poly-Ubiquitin, K63-linked (0.6 ug). Load: 5 uL. Block: 1% BSA in TBST. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:2000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | BAC56955.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Ubiquitin Antibody (PerCP) (orb699408)
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