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Ubiquityl-Histone H2B (Lys120) Recombinant Mouse Monoclonal Antibody
SKU: orb1172813
Description
Research Area
Epigenetics & Chromatin
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG2a, Kappa |
| Clone No. | A12B4 |
| Target | H2BC3 |
| Purification | Antigen affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H2BC3 | Histone H2B (ub-K120); ub-Histone H2B; ubiquityl-Histone H2B; H2BK120Ub; ub-H2B; H2B.1; H2B/f; H2BFF; HIST1H2BB; H2B1B_HUMAN; H2BC3; H2B-clustered histone 3; Histone H2B.1; Histone H2B.f (H2B/f);
Quality Guarantee
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HeLa, HepG2, Neuro-2a, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 23 kDa, Observed MW: 23 kDa.
Tissue: Human liver, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172813.
Tissue: Mouse kidney, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172813.
Tissue: Rat liver, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172813.
Quick Database Links
Gene Symbol
H2BC3
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Ubiquityl-Histone H2B (Lys120) Recombinant Mouse Monoclonal Antibody (orb1172813)
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