UCHL1 Antibody (C-term)

Fluorescent image of U251 cell stained with UCHL1 Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UCHL1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). UCHL1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

UCHL1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UCHL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

UCHL1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UCHL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

UCHL1 Antibody (C-term) western blot analysis in U266, NCI-H1299, mouse Neuro-2a cell line and mouse brain tissue lysates (35 ug/lane). This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).