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Urease Antibody Peroxidase Conjugated
SKU: orb344912
Description
Images & Validation
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| Tested Applications | DOT, ELISA, WB |
|---|---|
| Dilution range | ELISA: 1:500 - 1:2,000, WB: 1:50 - 1:500 |
| Reactivity | Plant |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Urease [Jack Bean] |
| Purity | Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean]. Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined. |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Concentration | 1.0 mg/ml |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Urease Antibody HRP Conjugation, Peroxidase Conjugated rabbit anti-Urease Antibody, Urea Amidohydrolase antibody
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Dot Blot Results of Rabbit Anti-Urease Antibody Peroxidase Conjugated. Urease (1) 33.33 ng, (2) 11.11 ng, (3) 3.70 ng, (4) 1.23 ng. Primary Antibody: Anti-Urease HRP at 1.0 µg/ml for 1hr at RT. Secondary Antibody: none. Blocking: BlockOut Buffer (orb348644) for 30 mins RT. Exposure: 5 secs.
Quick Database Links
UniProt
RefSeq:AAA83831.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | AAA83831.1 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
Urease Antibody Peroxidase Conjugated (orb344912)
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