Vazyme - Annexin V-PE/7-AAD Apoptosis Detection Kit

Using phycoerythrin (PE) to label Annexin V. The labeled Annexin V retains a high affinity for PSand can be used as a probe to detect apoptosis by fluorescence microscopy or flow cytometry.Since PS eversion occurs in the early stage of apoptosis, Annexin V-PE staining can identify theoccurrence of apoptosis in the early stage of apoptosis. 7-Aminoactinomycin D (7-AAD) is anucleic acid dye and cannot penetrate the intact cell membrane of normal cells or early apoptoticcells. However, 7-AAD can penetrate membrane-damaged cells such as late apoptotic cells ornecrotic cells, and bind to their DNA. It can be used to distinguish early apoptotic cells from lateapoptotic cells or necrotic cells. Therefore, by co-staining Annexin V-PE with 7-AAD, cells indifferent apoptotic stages can be distinguished. On the scattergram of dual-color flow cytometry,Annexin V-PE and 7-AAD double negative are normal cells, Annexin V-PE positive and 7-AADnegative are early apoptotic cells, and Annexin V-PE and 7-AAD double positive cells are lateapoptotic cells or necrotic cells.