Vazyme - ClonExpress II One Step Cloning Kit

The ClonExpress technology is a simple, fast, and highly efficient DNA seamless cloningtechnology. It enables directional cloning of inserts into any site in any vector. Use any methodto linearize the vector, and introduce the end sequence of the linearized vector at the 5' end ofthe insert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR producthave the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCRproduct and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 37°Cfor 30 min to complete the directionalcloning. ClonExpress II is a new generation of recombinant cloning kit. It has a unique ligase-independent system, which significantly reduces the self-ligation background of the vector, and the positiverate can reach more than 95%. Highly optimized reaction buffer and enhanced recombinaseExnase II can significantly improve the recombination efficiency and the tolerance to impurities.These features make it possible to use linearized vectors and inserts directly for recombinantcloning without purification, which significantly simplifies the experimental steps.