Vazyme - ClonExpress Ultra One Step Cloning Kit V2

The ClonExpress technology is simple, fast, and highly efficient DNA seamless cloning technology.It enables rapid directional cloning of inserts into any site in any vector. Use any method tolinearize the vector, and introduce the end sequence of the linearized vector at the 5' end of theinsert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR product havethe same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR productwith the same sequence as the end of the vector and the linearized vector are mixed in a certainproportion. Under the catalysis of recombinase, the transformation can be performed at 50°C for5 - 30 min to complete the directional cloning. ClonExpress Ultra One Step Cloning Kit V2 is a new generation of recombinant cloning kits,compatible with 1 - 5 fragments homologous recombination. Highly optimized 2 × CE Mix furthersignificantly improves the recombination efficiency of multiple fragments. This product hasbroader compatibility with the GC content of homology arms, which better guarantees thesuccess rate of cloning in more difficult application scenarios.