Vazyme - DNase I, RNase-free

Deoxyribonuclease I (DNase I) is an endonuclease that digests single- or double-stranded DNA. It recognizes and cleaves phosphodiester bonds to produce a single deoxynucleotide or single- or double-stranded oligodeoxynucleotide with 5'-phosphorylated and 3'-hydroxylated ends. The activity of DNase I depends on Ca 2+ , and DNase I can also be activated by divalent metal ions such as Mg 2+ and Mn 2+ . In the presence of Mg 2+ , DNase I nonspecifically recognizes and cleaves a double-stranded DNA at any site on either strand, and in the presence of Mn 2+ , it recognizes and cleaves almost the same sites on both strands of the DNA to produce DNA fragments with blunt ends or sticky ends with one or two nucleotide overhangs. Applications It is applicable for RNA extraction, in vitro transcription, DNA removal in RT-PCR, DNase I footprinting, nick translation, DNA library preparation and other molecular biological experiments.