Vazyme - Dual Luciferase Reporter Assay Kit

The Dual Luciferase Reporter Assay Kit is used to detect gene regulation by transfecting cells with a reporter plasmid and measuring thefluorescence intensity of Luciferin substrate to reflect the level of Luciferase expression. To achieve dual luciferase reporter genedetection, Firefly luciferase is detected with Luciferin as a substrate, and Renilla Iuciferase is detected with coelenterazine as a substrate,while inhibiting the activity of Firefly luciferase. Dual Luciferase Reporter Assay Kit can detect the expression of Luciferase regulated bygene elements sensitively and efficiently. Usually, the transcriptional regulatory element is cloned upstream of Firefly luciferase, or the3'-UTR regulatory region is cloned downstream of firefly luciferase. The transfected cells are induced by corresponding stimulator andlysed to determine the luciferase activity. The stimulatory-inducing effect of the regulatory elements is evaluated by luciferase activity.Renilla Iuciferase acts as an internal reference for correcting transfection efficiency to eliminate differences in cell number and transfectionefficiency between wells. Firefly luciferase catalyzes the emission of Luciferin at 560 nm, and Renilla luciferase catalyzes the emission ofcoelenterazine at 465 nm.