Vazyme - miRNA Unimodal SYBR qPCR Master Mix

This product is a specialized premix designed for miRNA quantification using the SYBR Green I fluorescence method. The premix is dyed blue for convenient and accurate pipetting. Due to the short length of miRNA sequences and the high similarity often found within miRNA families, achieving high specificity is critical for accurate quantification. The core enzyme of this product has been selectively developed through the BioSmart platform. It features exceptional specificity, strong 3'-end mismatch recognition, and the ability to extend the shortest possible base sequences. Combined with high-sealing dual-species antibodies, the enzyme forms a hot-start Taq polymerase that ensures strict activity suppression at 55°C. Paired with an optimized Buffer specifically designed for qPCR, this product delivers high sensitivity and specificity for miRNA quantification. This premix also includes a specially formulated ROX Passive Reference Dye, compatible with a wide range of qPCR instruments. No additional adjustments to ROX concentration are required across different platforms. Simply add primers and templates to the reaction system to begin amplification. In addition, this product provides universal U6 internal reference primers for human, rat, and mouse samples, enabling the generation of excellent standard curves over a broad dynamic range. For optimal performance, we recommend using this premix with themiRNA 1st Strand cDNA Synthesis Kit (by stem-loop) ( Vazyme #MR101 ) or themiRNA 1st Strand cDNA Synthesis Kit (by tailing A) ( Vazyme #MR201 ).