Vazyme - Mut Express II Fast Mutagenesis Kit V2

Mut Express II Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on theClonExpress homologous recombination technology for introducing single or double pointmutations in plasmids. The kit integrates the Phanta Max Super-Fidelity DNA Polymeraseamplification system and the ClonExpress rapid cloning system. With superior fidelity, PhantaMax significantly reduces the possibility of introducing novel mutations during amplification. Itsexcellent long fragment amplification ability is widely applicable to the amplification of anyplasmid less than 20 kb. The ClonExpress rapid cloning system replaces the conventionalannealing-cyclization reactions with the more efficient homologous recombination reaction.Site-directed mutagenesis using the Mut Express II Fast Mutagenesis Kit V2 allows a moreflexible primer design and extremely low template input, thereby facilitating complete degradation of the initial methylated template. The highly optimized recombination reaction buffer andenhanced recombinase Exnase II significantly improve the efficiency of recombinant cyclizationand tolerance to impurities. If the amplification product is highly specific, its Dpn I-digestedproduct can be used directly in the recombination reaction without DNA purification.