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Vazyme - SupRealQ Ultra Hunter SYBR qPCR Master Mix (U+)
SKU: Q713-03
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−| Storage | Store at -30 ~ -15°C and protect from light. Ship at ≤0°C. Performance C ompatibility with Complex Samples Through the BioSmart platform, our core enzyme is screened for high amplification performance and paired with a patented dual-species antibody blocking technology. This combination ensures stable amplification of complex templates, reducing the need for repeated system adjustments and enhancing researchers’ experimental efficiency. a. Reliable Detection of Relatively Low-Abundance Genes Using SupRealQ Ultra Hunter SYBR qPCR Master Mix (U+) (Vazyme #Q713), qPCR reactions were performed on 293T cDNA for various genes under identical conditions. Certain genes exhibited delayed C T values compared to others (as shown for Gene 1, Gene 2, and Gene 3 in the graph below), indicating their classification as relatively low-abundance genes. For Gene 3, amplification was carried out using Vazyme #Q713 and a competitor's dye-based qPCR reagent (Supplier A). Results demonstrated that Vazyme #Q713 outperformed Supplier A in sensitivity for low-abundance genes , while maintaining high specificity for amplification products. b. Amplification of Moderately Degraded Samples RNA extracted from the pancreas tissue of Litopenaeus vannamei was subjected to gel electrophoresis, which showed no distinct bands, indicating sample degradation. The degraded RNA was reverse-transcribed into cDNA, which was subsequently diluted in a 2-fold series across three gradients. Amplification of the shrimp gene was performed using Vazyme #Q713 and Supplier A's dye-based qPCR reagent on the ABI QuantStudio 3 system. The results showed that Vazyme #Q713°Chieved stable amplification of degraded samples , with amplification efficiency meeting the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guideline standard of 90-110%. c. Amplification of Genes with High and Low GC Content Using 293T gDNA as the template, Vazyme #Q713 and Supplier A's dye-based qPCR reagent were used to amplify Gene 4 and Gene 5, representing high and low GC content, respectively, on the ABI QuantStudio 3 system. The results demonstrated that Vazyme #Q713 exhibited superior sensitivity and specificity compared to Supplier A for both high- and low-GC content genes. Wide Linear Range Mouse liver cDNA was subjected to a 10-fold serial dilution, and qPCR amplification of mouse genes was performed using Vazyme #Q713 and Supplier A's dye-based qPCR reagent on the ABI QuantStudio 3 system. The results showed that Vazyme #Q713 exhibited amplification efficiency within the 90-110% range deemed reliable by MIQE guidelines across a wide linear range, outperforming Supplier A. Efficient Anti-Contamination Vazyme #Q713 incorporates a dUTP/Heat-labile UDG anti-contamination system, which effectively removes contaminants from the reaction mixture at room temperature. As the reaction temperature increases to 50-55°C, Heat-labile UDG rapidly inactivates, preserving the integrity of cDNA and ensuring that detection sensitivity remains unaffected. To test the contaminant removal efficiency, 60 pg and 600 pg of U-containing templates were added to the reaction mixture. The results showed that Vazyme #Q713°Chieved a contaminant removal efficiency of over 99.99% , effectively ensuring the accuracy of experimental results. |
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| Disclaimer | For research use only |
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Vazyme - SupRealQ Ultra Hunter SYBR qPCR Master Mix (U+) (Q713-03)
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