Vazyme - VAHTS Library Quantification Kit for Illumina 2.0

VAHTS Library Quantification Kit for Illumina 2.0 is for absolute, dye-based qPCR quantification of Illumina libraries. The principle is to generate the standard curve using each DNA Standard, then the concentrations of diluted library samples are calculated against the standard curve using absolute quantification. This kit utilizes a novel dye-based qPCR mix VAHTS SYBR qPCR Master Mix 2.0 based on the antibody-mediated hot start DNA polymerase. This component completely premixes amplification reagent, primers and ROX, which has many advantages such as strong specificity, high amplification efficiency, good GC content compatibility, high sensitivity, easy operation and rapid amplification. This kit is therefore suitable for high-throughput sample quantification. All reagents provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability of different lots.