VCAM1 Rabbit Polyclonal Antibody

SKU: orb11550

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Description

Research Area

Cardiovascular, Cardiovascular Research, Cell Adhesion, Cell Biology, Cell Markers, Cell adhesion Molecules, Endothelial, Molecular Biology, SARS /COVID-19, Signal Transduction, Surface Molecules, Varscular, Varscular System

Images & Validation

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Item 1 of 5

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Mouse
Predicted Reactivity	Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Mouse VCAM 1 protein
Target	VCAM1
Molecular Weight	81 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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VCAM1; CD 106; CD106; CD106 Antigen; DKFZp779G2333; INCAM 100; L1CAM; MGC99561; Vascular Cell Adhesion Molecule 1; Vascular cell adhesion protein 1; VCAM 1; VCAM-1; INCAM-100; V-CAM 1; VCAM1_HUMAN.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Blank control: Mouse Spleen (blue). Primary Antibody: Rabbit Anti-VCAM-1 antibody (orb11550, Green), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde for 10 min at 37°C. Primary antibody (orb11550, 1 µg/8x10^5 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (1 hour) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Mouse Cerebrum tissue lysates, Primary: Anti-VCAM1 (orb11550) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kDa, Observed band size: 100 kDa.

Sample: Lane 1: Siha (Human) Cell Lysate at 30 ug, Lane 2: Huvec (Human) Cell Lysate at 30 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-VCAM-1 (orb11550) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110 kD, Observed band size: 110 kD.

Sample: VCAM1 protein (Human) at 100 ng, Primary: Anti-VCAM-1 (orb11550) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-VCAM-1 Polyclonal Antibody, Unconjugated (orb11550) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

VCAM1

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Protocol Information

Western Blot (IB, immunoblot)

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Find More Protocols

Cao, Wei et al. Reducing-Autophagy Derived Mitochondrial Dysfunction during Resveratrol Promotes Fibroblast-Like Synovial Cell Apoptosis Anat Rec (Hoboken), 301, 1179-1188 (2018)

PubMed: 29461680