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VDAC1/Porin Antibody
SKU: orb1294402
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:400 Immunohistochemistry (Paraffin) 1:200-1:300 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the center region. |
| Target | VDAC1 / Porin |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Anti-VDAC1 beta antibody - at 1/500 dilution. Lysates at 60 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294402 overnight at 4°C. Asterisk indicates a 53kDa band of unknown identity detected by this antibody in Western blot analysis.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294402 anti-VDAC1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling VDAC1 with orb1294402 at 1/150.
Quick Database Links
Gene Symbol
VDAC1 / Porin
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
VDAC1/Porin Antibody (orb1294402)
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