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Vegfa Antibody

SKU: orb1272689

Description

Vegfa Antibody

Research Area

Cardiovascular Research; Cell Biology

Images & Validation

Tested ApplicationsELISA, NeA, WB
ReactivityRat
Application Notes
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of Rat VEGF (10 ng/mL), a concentration of Indirect:To detect Rat VEGF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF.SandwichTo detect Rat VEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat VEGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF. Western Blot:To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
ImmunogenProduced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat VEGF (Rat Vascular Endothelial Growth Factor). Rat VEGF specific antibody was purified by affinity chromatography employing immobilized Rat VEGF matrix.
TargetVegfa
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

VPF, Vegf, VEGF-A, VEGF164, Vascular endothelial growth factor A, Vascular permeability factor

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP16612

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 780.00
Free Secondary Antibody (20 ul)0/0

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