VEGF Rabbit Polyclonal Antibody

SKU: orb1294346

Description

Rabbit polyclonal antibody to VEGF

Research Area

Cardiovascular Research

Images & Validation

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Item 1 of 14

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:500–1:1000, Immunofluorescence 1:200–1:500, Immunohistochemistry (Paraffin) 1:100–1:300, Immunoprecipitation : Reported in the literature, not verified in house.
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / encompassing a sequence within the center region
Target	VEGF
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294346 anti-VEGF antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling VEGF with orb1294346 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling VEGF with orb1294346 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Kidney cancer tissue labeling VEGF with orb1294346 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Liver and Testis cancer tissue labeling VEGF with orb1294346 at 1/100.

Immunohistochemical analysis of paraffin embedded mouse colon labeling VEGF with orb1294346 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse Lung labeling VEGF with orb1294346 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

VEGF antibody at 1/1000 dilution. Lysates/proteins at 35 µg per lane. This blot was produced using a 15% SDS-PAGE.

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Gene Symbol

VEGF

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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