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VEGF Antibody
SKU: orb1294346
Description
Research Area
Cardiovascular Research
Images & Validation
−Item 1 of 14
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:500–1:1000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100–1:300 Immunoprecipitation : Reported in the literature, not verified in house. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the center region |
| Target | VEGF |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294346 anti-VEGF antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling VEGF with orb1294346 at 1/100.
Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling VEGF with orb1294346 at 1/100.
Immunohistochemical analysis of paraffin embedded Human Kidney cancer tissue labeling VEGF with orb1294346 at 1/100.
Immunohistochemical analysis of paraffin embedded Human Liver and Testis cancer tissue labeling VEGF with orb1294346 at 1/100.
Immunohistochemical analysis of paraffin embedded mouse colon labeling VEGF with orb1294346 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse Lung labeling VEGF with orb1294346 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
VEGF antibody at 1/1000 dilution. Lysates/proteins at 35 µg per lane. This blot was produced using a 15% SDS-PAGE.
VEGF antibody at 1/1000 dilution. Lysates/proteins at 35 µg per lane. This blot was produced using a 15% SDS-PAGE.
Quick Database Links
Gene Symbol
VEGF
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
VEGF Antibody (orb1294346)
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