SLC18A2 Antibody

SKU: orb1247892

Description

SLC18A2 Antibody

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Porcine, Rat
Application Notes	Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Placenta and Adrenal Gland lysates (calculated MW of 55.7kDa according to NP_003045.2). Recommended concentration 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 5ug/ml. In paraffin embedded Human Adrenal Gland expression of SLC18A2 is detected in chromaffin cells. Recommended concentration: 2.5ug/ml Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Immunogen	The immunogen for this antibody is: C-SYPIGEDEESESD
Target	SLC18A2
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Disclaimer	For research use only

Alternative Names

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SLC18A2, VMAT2, SVAT, SVMT, VAT2, solute carrier family 18 (vesicular monoamine), member 2, MGC120477, MGC120478, MGC26538

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orb1247892 (1 ug/ml) staining of Human Placenta (A) and Human Adrenal Gland (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247892 (5 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247892 (2.5 ug/ml) staining of paraffin embedded Human Adrenal Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1247892 Negative Control showing staining of paraffin embedded Human Adrenal Gland, with no primary antibody.

Quick Database Links

Gene Symbol

SLC18A2

UniProt

Q05940

RefSeq (Protein):NP_003045.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003045.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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