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VPS35 retromer complex component Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ICC, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:10000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary. |
| Target | VPS35 |
| Molecular Weight | 92 |
| Purity | Purified by antigen-affinity chromatography. |
| Purification | Purified by antigen-affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Buffer/Preservatives | PBS, 20% Glycerol, 0.025% ProClin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−VPS35 Antibody (Biotin) [orb612801]
ICC, IF, IHC, IP, WB
Human, Mouse, Rat
Mouse
Monoclonal
Biotin
100 μg

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Immunofluorescence analysis of HeLa cells using VPS35 antibody

Immunohistochemical staining of human colon carcinoma using VPS35 antibody

Western blot analysis of H1299(Lane 1), HeLa S3(Lane 2), Hep G2(Lane 3), MOLT4(Lane 4), Raji(Lane 5) using VPS35 antibody

Western blot analysis of H1299(Lane 1), HeLa S3(Lane 2), Hep G2(Lane 3), MOLT4(Lane 4), Raji(Lane 5) using VPS35 antibody

Western blot analysis of H1299(Lane 1), HeLa S3(Lane 2), Hep G2(Lane 3), MOLT4(Lane 4), Raji(Lane 5) using VPS35 antibody

Western blot analysis of H1299(Lane 1), HeLa S3(Lane 2), Hep G2(Lane 3), MOLT4(Lane 4), Raji(Lane 5) using VPS35 antibody
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Protocol Information
VPS35 retromer complex component Antibody (orb556484)
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