XBP1 Antibody

Immunofluorescence Validation of XBP-1 in HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling XBP-1 with orb1240297 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

XBP-1 KO Validation in 293T Cells. Loading: 10 µg of lysate Antibodies: XBP-1 orb1240297, 4 µg/mL and beta-actin, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 100 ng of human XBP-1 recombinant protein per lane. Antibodies: XBP-1 orb1240297 (A: 0.5 µg/mL, B: 1 µg/mL and C: 2 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 23kD.

Western Blot Validation of XBP-1 in HepG2 Cells. Loading: 15 µg of HepG2 cell lysate Antibodies: XBP-1 orb1240297, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane1: 1 µg/mL Lane2: 2 µg/mL.

Western Blot Validation in Mouse Pancreas Tissue. Loading: 15 µg of lysates per lane. Antibodies: XBP-1, orb1240297 (4 µg /mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Liver Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: XBP-1 orb1240297 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. (1) the absence and (2) the presence of blocking peptide.

Immunofluorescence Validation of XBP-1 in Human Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling XBP-1 with orb1240297 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of XBP-1 in Human Liver Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Liver Tissue labeling XBP-1 with orb1240297 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of XBP-1 in Mouse Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling XBP-1 with orb1240297 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of XBP-1 in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-XBP-1 antibody (orb1240297) at 1 µg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of XBP-1 in Mouse Spleen Tissue. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-XBP-1 antibody (orb1240297) at 1 µg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of XBP-1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-XBP-1 antibody (orb1240297) at 1 µg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.