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Y14 Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | ELISA, FC, ICC, IHC-P, IP, WB |
|---|---|
| Dilution range | Optimal antibody dilution should be determined by titration, however as a guideline try; Flow Cyt: Use 1-2µg for 106 cells IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ICC/IF: 1/15. PubMed: 17103222 WB: Use at an assay dependent dilution. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). PubMed: 17103222 |
| Reactivity | Human, Xenopus |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | 4C4 |
| Immunogen | Full length native protein (purified) (Human) |
| Target | Y14 |
| Purification | Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS 0.1% sodium azide |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
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Confocal immunofluorescence analysis of paraffin-embedded HeLa cells using Y14 antibody
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Y14 Monoclonal Antibody (orb76831)
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