Z-FA-FMK

SKU: orb1300512

Description

Z-FA-FMK

Research Area

Cell Biology, Infectious Disease & Virology, Protein Biochemistry

Images & Validation

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Key Properties

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CAS Number	197855-65-5
MW	386.42
Purity	99.43% (May vary between batches)
Formula	C₂₁H₂₃FN₂O₄
SMILES	CC(NC(=O)[C@H](Cc1ccccc1)NC(=O)OCc1ccccc1)C(=O)CF
Target	Cysteine Protease\|\|\|SARS-CoV
Solubility	10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.18 mM);DMSO:71 mg/mL (183.74 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:32 mg/mL (82.81 mM)

Bioactivity

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Target IC50	3CLpro (SARS Protease):25.7 μM (Ki)
In Vivo	Z-FA-FMK effectively inhibits T-cell proliferation induced by interleukin-2 (IL-2) and mitogens in vitro. It also prevents the degradation of fibrillar collagen through its actions on fibroblasts and osteoclasts. Furthermore, Z-FA-FMK suppresses the expression of NF-κB-dependent genes in macrophages, thereby inhibiting the production of cytokines induced by lipopolysaccharides.
In Vitro	Z-FA-FMK inhibits the development of Ras-induced carcinogenic tumors and respiratory viral infections in the cardiac tissue of mice with severe combined immunodeficiency (SCID). In a mouse model of intranasal pneumococcal infection, Z-FA-FMK significantly promotes the growth of Streptococcus pneumoniae in both the lungs and the bloodstream.
Cell Research	T cell proliferation following mitogen stimulation is determined using [3H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-3H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester. (Only for Reference)

Storage & Handling

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Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Caspase, Apoptosis, Cysteine protease, CysteineProtease, Cysteine Protease, DNA fragmentation, DEVDase, Cathepsin, inhibit, Inhibitor, externalization of phosphatidylserine, ZFAFMK, Z-FA-FMK, Z FA FMK, reovirus replication, RRMs, SARS-CoV, SARSCoV, SARS coronavirus

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Compound Identifiers

PubChem CID

6915837 ↗

Key Properties

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No computed properties available.

Protocol Information

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Z-FA-FMK

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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