ZAP-70 antibody

SKU: orb2276361

Description

ZAP-70 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

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Item 1 of 3

Tested Applications	IHC
Dilution Range	1:75-1:150
Reactivity	Human
Application Notes	Positive Control: Tonsil: A large fraction of the lymphocytes in the interfollicular areas and few lymphocytes in the germinal centres should show a strong ZAP70 immunostaining. Negative Control: Tonsil: ZAP70 immunostaining should be absent in epithelial tissues and the majority of cells in germinal centres. Cellular Localization: Cell Surface and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. Read more...

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone No.	MSVA-870R
Immunogen	Recombinant fragment (around aa 247-382) ofhuman ZAP70 protein (exact sequence is proprietary)
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Selective T cell defect; SRK; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70;TZK; Zeta chain associated protein kinase 70kDa

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B CLL with strong ZAP70 staining of tumor cells and even stronger staining in interspersed normal T lymphocytes.

In lymph nodes ZAP70 positive lymphocytes predominate in the interfollicular zone but are much less numerous in germinal centres.

ZAP70 negative B CLL containing a significant number of ZAP70 positive T lymphocytes.

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UniProt

P43403

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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ZAP-70 antibody (orb2276361)

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Applications:	FC, ICC, WB
Reactivity:	Human
Clonality:	Monoclonal
Conjugation:	Unconjugated

Applications:	ELISA, IHC, WB
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Monoclonal
Conjugation:	Unconjugated

Applications:	FACS, IF, IHC-P, WB
Reactivity:	Human
Species/Host:	Mouse
Clonality:	Monoclonal
Conjugation:	Unconjugated

Applications:	FACS, IF, IHC-P, WB
Reactivity:	Human
Species/Host:	Mouse
Clonality:	Monoclonal
Conjugation:	Unconjugated

Applications:	FC, IF, WB
Reactivity:	Human, Mouse
Species/Host:	Mouse
Clonality:	Monoclonal
Conjugation:	Unconjugated

ZAP-70 antibody

Description

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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UniProt Details

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Protocol Information

ZAP-70 antibody (orb2276361)

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