You have no items in your shopping cart.
ZAP-70 Antibody
SKU: orb749850
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | FACS, IF, IHC-P, WB |
|---|---|
| Dilution Range | Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a, kappa |
| Clone No. | 2F3.2 |
| Immunogen | Recombinant human protein (including amino acids 1-254 and the SH2 domains) was used as the immunogen for the ZAP-70 antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Similar Products
−- Item 1 of 4
- Item 1 of 3
- Item 1 of 3
ZAP70 Antibody [orb1410176]
ELISA, IHC, WB
Human
Rabbit
Monoclonal
Unconjugated
20 μg, 100 μg, 100 μg (without BSA and Azide) - Item 1 of 5
- Item 1 of 3
Phospho-ZAP70 (Tyr292) Rabbit Polyclonal Antibody [orb158734]
FC, WB
Bovine, Canine, Equine, Porcine, Rat
Human, Mouse
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Immunofluorescent staining of PFA-fixed human Jurkat cells with ZAP-70 antibody (green, clone 2F3.2) and Reddot nuclear stain (red).
IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with ZAP-70 antibody (clone 2F3.2).
Western blot testing of human Jurkat cell lysate with ZAP-70 antibody (clone 2F3.2).
Flow testing of PFA-fixed Jurkat cells with ZAP-70 antibody (clone 2F3.2); Red = isotype control, Blue = ZAP70 antibody.
SDS-PAGE analysis of purified, BSA-free ZAP-70 antibody (clone 2F3.2) as confirmation of integrity and purity.
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
ZAP-70 Antibody (orb749850)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days