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ZIC3 Antibody
SKU: orb1264617
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This ZIC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the Central region of human ZIC3. |
| Target | ZIC3 |
| Molecular Weight | 51 kDa |
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Zinc finger protein ZIC 3, Zinc finger protein 203, Zinc finger protein of the cerebellum 3, ZIC3, ZNF203
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Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in CEM cell line lysates (35 ug/lane).
ZIC3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ZIC3 Antibody (orb1264617)
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