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2-Hydroxyisobutyryl-Histone H3 (Lys14) Recombinant Rabbit Monoclonal Antibody

SKU: orb1173116

Description

2-Hydroxyisobutyryl-Histone H3 (Lys14) Recombinant Rabbit Monoclonal Antibody

Images & Validation

Tested ApplicationsWB
Dilution rangeWB=1:500-2000
ReactivityHuman, Mouse, Rat
Predicted ReactivityMouse, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG
Clone No.B3E9
TargetH3C1
PurificationAffinity purified by Protein A

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

2-Hydroxyisobutyryl-Histone H3 (Lys14) Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + sodium butyrate (30mM, 4hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 15 kDa, Observed MW: 15 kDa.

2-Hydroxyisobutyryl-Histone H3 (Lys14) Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 15 kDa, Observed MW: 15 kDa.

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Protocol Information

WB
Western Blot (IB, immunoblot)
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2-Hydroxyisobutyryl-Histone H3 (Lys14) Recombinant Rabbit Monoclonal Antibody (orb1173116)

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25 μl
¥ 2,210.00
50 μl
¥ 4,160.00
100 μl
¥ 5,980.00
DispatchUsually dispatched within 5-10 working days
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