A20 Antibody / TNFAIP3

SKU: orb699507

Description

A20 (also known as TNFAIP3) is a zinc finger protein and inhibits NF-kappa B activation as well as TNF-mediated apoptosis. The protein has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. A20 is as a tumor suppressor and has been shown to protect MCF-7 breast carcinoma cells from TNF-induced apoptosis. High A20 expression levels were observed in more aggressive breast tumors (ER-negative, progesterone receptor-negative and high histological grade). These findings strongly suggest that A20 is a key protein involved in tamoxifen resistance.

Research Area

Apoptotic, Cancer, Epigenetics, Immunology, Neuroscience, Signaling Pathways

Images & Validation

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Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Reactivity	Human
Application Notes	Optimal dilution of the A20 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2c, kappa
Clone No.	TNFAIP3/2813
Immunogen	A portion of amino acids 241-356 from the human protein was used as the immunogen for the A20 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human breast carcinoma with A20 antibody (clone TNFAIP3/2813). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human hepatocellular carcinoma with A20 antibody (clone TNFAIP3/2813). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P21580

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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