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ABCB5 Antibody (N-term)
SKU: orb1930242
Description
Research Area
Cancer Biology, Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 7
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | FC - 1:25, WB - 1:1000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 138641 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Overlay histogram showing HepG2 cells stained (green line). The cells were fixed with 2% paraformaldehyde 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
Western blot analysis of ABCB5 Antibody (N-term) in A375, K562, A2058 and HL-60 cell line lysates (35 ug/lane). ABCB5 (arrow) was detected using the purified Pab.
Flow Cytometry using ABCB5 Antibody (N-Term) on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer.
Flow Cytometry using ABCB5 Antibody (N-Term) on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer.
Anti-ABCB5 Antibody (N-term) at 1:2000 dilution + Mouse eyeball lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 138 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-ABCB5 Antibody (N-term) at 1:2000 dilution. Lane 1: A375 whole cell lysate. Lane 2: HL-60 whole cell lysate. Lane 3: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 138 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-ABCB5 Antibody (N-term) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: A375 whole cell lysate. Lane 5: A2058 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 138 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ABCB5 Antibody (N-term) (orb1930242)
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