Vazyme - Add&Read Human IL7 Quantitative Detection Kit

Interleukin-7 (IL-7) is a pleiotropic cytokine primarily produced by bone marrow stromal cells. Its main functions include promoting the growth of B and T cells and exerting anti-apoptotic effect s. It plays a crucial role in the growth, survival, and differentiation of T cells, while also exhibiting potential chemotactic activity. Like other cytokines, IL-7 is an immunoregulatory protein that plays an important role in the normal development of the immune system and the maintenance of normal immune function. This kit uses a sandwich assay to detect IL-7 levels. The kit includes IL-7 Standard and two monoclonal antibodies specifically recognizing IL-7. One antibody is conjugated with Eu (donor, Anti-IL-7-Eu), and the other is conjugated with A3 (acceptor, Anti-IL-7-A3). When both antibodies bind to IL-7 simultaneously, Anti-IL-7-Eu and Anti-IL-7-A3 come into close proximity, enabling fluorescence resonance energy transfer (FRET). The fluorescence donor is excited by light at 320/340 nm, emitting light at 620 nm. This 620 nm light then excites the fluorescence acceptor, which emits light at 665 nm. The concentration of IL-7 in the sample is proportional to the FRET signal value (the ratio of light intensity at 665 nm to 620 nm).