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ADGRE1 Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | IF, IHC-Fr, IHC-P |
|---|---|
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Mouse |
| Predicted Reactivity | Guinea pig, Porcine, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human ADGRE1 (701-800/886aa) |
| Target | ADGRE1 |
| Molecular Weight | 95 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−ADGRE1 Antibody [orb626873]
ELISA, IF, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μg, 50 μgADGRE1 Rabbit Polyclonal Antibody [orb1816982]
FC
Mouse
Mouse
Rabbit
Polyclonal
Unconjugated
50 μl, 200 μl, 100 μl

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Blank control (black line): Raw264.7. Primary Antibody (green line): Rabbit Anti-ADGRE1 antibody (orb156726), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Recombinant human EMR1 protein, N-His, Primary: Anti-ADGRE1 (orb156726) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 95 kDa, Observed band size: 32 kDa.
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UniProt Details
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Protocol Information
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Xuxin Chen 1, Fan Wang 1, Jian Tang 1, Jiguang Meng 2, Zhihai Han 3 Paralemmin-3 augments lipopolysaccharide-induced acute lung injury with M1 macrophage polarization via the notch signaling pathway Respir Physiol Neurobiol, 320, 104203 (2024)
Applications
Reactivity
Roham Deyhimfar 1, Keykavos Gholami 2, Iman Menbari Oskouie 1, Leila Zareian Baghdadabad 1, Parisa Zahmatkesh 1, Mohammadreza Shoghi 3, Gholamreza Mesbah 4, Fateme Guitynavard 1, Seyed Mohammad Kazem Aghamir 5 High-dose vitamin C improves BCG immunotherapy's efficacy in a murine ectopic model of bladder cancer Pathol Res Pract ., (2025)
Applications
Reactivity
ADGRE1 Rabbit Polyclonal Antibody (orb156726)
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